Last edited by Kigore
Wednesday, July 15, 2020 | History

5 edition of mRNA Processing and Metabolism found in the catalog.

mRNA Processing and Metabolism

Methods and Protocols (Methods in Molecular Biology)

by Daniel R. Schoenberg

  • 116 Want to read
  • 20 Currently reading

Published by Humana Press .
Written in English

    Subjects:
  • Biochemistry,
  • Messenger RNA,
  • Life Sciences - Biochemistry,
  • Science,
  • metabolism,
  • Medical,
  • Microbiology,
  • Life Sciences - Biology - Molecular Biology,
  • Science / Cytology,
  • Laboratory manuals,
  • Endocrinology & Metabolism,
  • Genetic Techniques,
  • RNA, Messenger

  • The Physical Object
    FormatHardcover
    Number of Pages288
    ID Numbers
    Open LibraryOL8828387M
    ISBN 101588292258
    ISBN 109781588292254

    Nucleic acid - Nucleic acid - Nucleic acid metabolism: Replication, repair, and recombination—the three main processes of DNA metabolism—are carried out by specialized machinery within the cell. DNA must be replicated accurately in order to ensure the integrity of the genetic code. Errors that creep in during replication or because of damage after replication . Prokaryotic mRNA processing is relatively unimportant in regulating gene expression. The chief function of prokaryotic mRNA seems to be to regulate stability. The terminator stem and loop stabilizes mRNA against nucleolytic degradation, and in some cases, removal of this structure destabilizes mRNA so that it is transcribed less efficiently.

      de Pretis, S. et al. INSPEcT: a computational tool to infer mRNA synthesis, processing and degradation dynamics from RNA- and 4sU-seq time course experiments. Bioinformat – (). The messenger RNA precursors (pre-mRNA) 3-end processing occurs in a two-step co-transcriptional coupled reaction, denoted as cleavage and polyadenylation. Both processes depend on trans-acting factors interacting in a coordinated manner with cis-sequence motifs located at the 3 untranslated region of transcripts.

    RNA metabolism and disease. Gene expression is a stepwise process involving distinct cellular processes including transcription, pre-mRNA processing, mRNA export, RNA trafficking, and translation. Recent studies have shown that the different steps in this gene regulation pathway are extensively coupled mechanistically by multifunctional factors.   Finally, I describe a role for the histone pre-mRNA processing factor SLBP in nuclear export of the mature mRNA. A detailed molecular analysis of the histone mRNA in cells with extremely low levels of SLBP reveals that the major lesion in histone mRNA metabolism is retention of mature, properly processed mRNA in the nucleus.


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MRNA Processing and Metabolism by Daniel R. Schoenberg Download PDF EPUB FB2

MRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and : Hardcover.

mRNA processing is a key step in gene expression that effects all the proteins within the cell. In mRNA Processing and Metabolism: Methods and Protocols, world-renowned researchers bring together the latest techniques spanning the breadth of mRNA processing and metabolism.

Antisense technology can be employed to modulate RNA metabolism and expression through several mechanisms (Crooke,and Table 1) including the modulation of RNA splicing (Condon and Bennett, ), the inhibition of mRNA translation into protein (Alt et mRNA Processing and Metabolism book, ; Baker, ; Brown-Driver et al., ; Zhang et al., ) the disruption of necessary RNA.

General description A collection of reproducible techniques for the study of mRNA processing and metabolism.

These techniques range from cotranscriptional processing events that occur while the mNA is engaged with elongating RNA polymerase II, to in vivo and in vitro splicing and its biochemical analysis, and alternative splicing.

Additional methods cover mRNA export, the. This book provides a collection of novel studies and hypotheses aimed to define the pathophysiological consequences of altered mRNA metabolism events in human cells, and is written for a wide spectrum of readers in the field of gene expression regulation.

Nucleic acid - Nucleic acid - RNA metabolism: RNA provides the link between the genetic information encoded in DNA and the actual workings of the cell. Some RNA molecules such as the rRNAs and the snRNAs (described in the section Types of RNA) become part of complicated ribonucleoprotein structures with specialized roles in the cell.

Others such as tRNAs play key. Messenger RNA (mRNA) carries the sequences that encode the amino acid sequence of one or more polypeptides specified by a gene or set of genes in the chromosomes. Transfer RNA (tRNA) is an adapter that reads the information encoded in the mRNA and transfers the appropriate amino acid to the growing polypeptide chain during protein synthesis.

All of a pre-mRNA’s introns must be completely and precisely removed before protein synthesis. If the process errs by even a single nucleotide, the reading frame of the rejoined exons would shift, and the resulting protein would be dysfunctional.

The process of removing introns and reconnecting exons is called splicing (Figure ). Introns. In molecular biology, messenger RNA (mRNA) is a single-stranded molecule of RNA that corresponds to the genetic sequence of a gene and is read by a ribosome in the process of synthesizing a protein.

mRNA is created during the process of transcription, where an enzyme (RNA polymerase) converts the gene into primary transcript mRNA (also known as pre-mRNA. " the shear diversity of current techniques on offer in this book makes it a valuable resource not only for those studying mRNA processing and metabolism but for those interested in RNA or RNA-protein interactions in general." - ChemBioChem Read more.

The rat cerebral cortex is a brain region known to be rich in CCK. The SK-N-MCIXC cell line provides an in vitro model to study the regulation of CCK synthesis and metabolism in neuronal systems since it contains the storage granules, mRNA, intact peptide, and complement of enzymes necessary for biosynthesis and metabolism of CCK.

RNA Metabolism Transcription - process by which DNA (genetic info) gets made into RNA (mRNA, rRNA, or tRNA) by an RNA polymerase mRNA - messenger RNA - encodes the amino acid sequence of > 1 protein specified by a gene(s) rRNA - ribosomal RNA - constituents of ribosomes (proteins synthesized here) - catalytic and directly involved in protein.

Interestingly, this analysis revealed that the AARGs are mostly involved in RNA-metabolism (transcription, processing, splicing, and oxidation of RNA), supporting our previous hypothesis on the. The roles of sumoylation in RNA metabolism, however, while considerable, remain less well understood.

In this chapter we have assembled data from proteomic analyses, localization studies and key functional studies to extend SUMO's role to the area of mRNA processing and metabolism. The three most important steps of pre-mRNA processing are the addition of stabilizing and signaling factors at the 5' and 3' ends of the molecule, and the removal of intervening sequences that do not specify the appropriate amino acids.

In rare cases, the mRNA transcript can be “edited” after it is transcribed. Pre-mRNA Splicing. The vast majority of mammalian pre-mRNAs contain introns that are removed by 2 trans-esterification reactions catalyzed by the spliceosome and associated auxiliary proteins that result in the removal of an intron and the joining of the 2 exons that border the intron (Figure 2).The core of the spliceosome is composed of a complex of 5 small nuclear.

mRNA METABOLISM & POST-TRANSCRIPTIONAL GENE REGULATION Edited by Joe B. Harford and David R. Morris Gene expression is a process that begins with the transcription ofDNA to an RNA messenger (mRNA), which is then translated into aprotein. Historically, attention has been focused on the regulationof RNA synthesis (transcription); however, there.

This process is called RNA processing. Figure 9: mRNA Processing in Eukaryotic Cells (Excision of Interons and Joining of Exons). During processing of the precursor mRNA, interons are excised and exons are joined together to produce a mature mRNA. GAPDH Binding to TNF-α mRNA Contributes to Posttranscriptional Repression in Monocytes: A Novel Mechanism of Communication Between Inflammation and Metabolism J Immunol.

Mar 15;(6) doi: /jimmunol metabolism. All chemical reactions. within organisms that enable them to maintain life. The two main categories of metabolism are catabolism and anabolism.

chemical energy. Energy contained in the. bonds between atoms of a molecule. photosynthesis. The process by which green plants use radiant energy from the sun to.

(mRNA Processing and Metabolism-Humana Press () كتاب يحمل اسم.The mRNA Metabolism in Human Disease / edited by Luísa Romão. Edition: 1st ed. Publication: Cham: Springer International Publishing: Imprint: Springer, Series: Biomedical and Life Sciences (Springer) Advances in experimental medicine and biology ; Advances in Experimental Medicine and Biology, ; Sterol regulatory element binding proteins (SREBPs) can regulate the lipid homeostasis by regulating its target genes, which are crucial for the cholesterol and fatty acid metabolism.

However, the transcriptional regulation role of SREBPs in fat-tailed sheep is unclear. In this study, two Chinese representative breeds of total 80 fat-tailed sheep were employed, serum .